23 Jul Preparation of polylactic-co-glycolic acid (PLGA) nano-fiber scaffold and application of PLGA nano-fiber scaffold to tissue engineering
Abstract
The invention discloses preparation of a polylactic-co-glycolic acid (PLGA) nano-fiber scaffold and application of the PLGA nano-fiber scaffold to tissue engineering, and belongs to the field of the tissue engineering. The method comprises the following steps of: preparing a fiber film containing two materials by taking PLGA and polyvinylpyrrolidone (PVP) as a raw material through electrostatic blended spinning, and dissolving and removing the PVP in an aqueous solution to obtain the PLGA nano-fiber scaffold with certain microstructures. A cell/PLGA nano-fiber scaffold composite matrix is obtained by the following steps of: inoculating seed cells on the PLGA nano-fiber scaffold, culturing in vitro, folding a cell/PLGA nano-fiber scaffold composite, and culturing for 7 to 14 days in a spinner cultivation mode. The composite matrix can be taken as a tissue engineering material with a specific tissue repair function and implanted into an animal model. The preparation process is simple and feasible, is high in repeatability, and can be widely applied to the field of the tissue engineering.
Year: 2013
Country: CN
Doc No: 102886068