23 Jul Preparation method of two-ion starch-based stem cell expansion hydrogel and stem cell expansion and collection method
Abstract
The invention discloses a preparation method of a two-ion starch-based stem cell expansion hydrogel and a stem cell expansion and collection method. The hydrogel is prepared from metacrylic acid estermodified two-ion starch and double-ended sulfydryl modified polyethylene glycol in sulfydryl-alkene click means, and CGRGDS polypeptides are introduced in the preparation process, so that the affinity of the hydrogel for cells is improved. The mechanical strength of the hydrogel can be controlled within the range of 27.21 to 69.92 kPa through the content of a polymer. More importantly, the adhesion of nonspecific proteins in the cell expansion process can be effectively reduced. The hydrogel has high biocompatibility, the multiplication of brown fat stem cells can be supported, and the stem cell characteristic is maintained in the multiplication process. Solid technical support can be provided for tissue engineering and cell treatment strategy. The hydrogel is chemically obtained throughclicking, the participation of an organic solvent is eliminated, the environment is protected, and the disadvantage that the support biocompatibility is reduced, which is caused in the synthetic process, is reduced.
Year: 2018
Country: CN
Doc No: 107936267