23 Jul Preparation method of tissue engineering cornea
Abstract
The invention relates to a preparation method of a tissue engineering cornea, comprising the following steps of: by using epidermal stem cells and pluripotent stromal stem cells as seed cells, planting on the two surfaces of an acellular natural cornea stroma after in-vitro amplification culture; and then forming the tissue engineering cornea through in-vitro induction culture. A scaffold used inthe preparation method not only overcomes the problem of serious immunological rejection of an xenogeneic cornea stroma, but also retains the structure (capable of recovering the transparency of the cornea) and main components (comprising a growth factor capable of promoting the growth, the proliferation and the differentiation of cornea cells) of a natural cornea. Compared with a product of the prior art, the invention has low cost, simple and convenient operation, extensive sources and easy storage; and in addition, the tissue engineering cornea containing living cells has certain elasticity and toughness, easy changes on shape, size and thickness and extremely low immunogenicity, can prevent complications caused by non-corneal materials and be rebuilt by recipient cells when implanted in vivo and rapidly integrated with an organism to realize transparency and is suitable for repairing corneal injury caused by various reasons.
Year: 2010
Country: CN
Doc No: 101757691