23 Jul Preparation method of tissue engineering cell sheet with osteogenic differentiation and vasculogenesis
Abstract
The invention discloses a preparation method of a tissue engineering cell sheet with osteogenic differentiation and vasculogenesis. The preparation method comprises the steps of firstly, separating BMSCs (bone marrow mesenchymal stem cells), carrying out passage multiplication culture of the BMSCs, and culturing mesenchymal stem cell sheet to obtain the tissue engineering cell sheet with osteogenic differentiation and vasculogenesis. The cell sheet technology is adopted to separate and culture BMSCs, the obtained BMSCs are of a complete sheet structure containing extracellular matrix and having osteogenic differentiation and vasculogenesis potency. As a tissue formed by pure cells is transplanted into a body, the use of a scaffold and other carriers and the untoward effects are avoided; and besides, the BMSCs cell sheet retains important cell surface protein such as an ion channel, ligandin and the like, intercellular signal transmission can be performed well, the functions are kept to be coordinated, and the cell utilization rate and the cell transferring activity are greatly improved.
Year: 2014
Country: CN
Doc No: 103773734