23 Jul Preparation method of three-dimensional porous antibacterial silk fibroin/graphene/titanium dioxide bone tissue engineering scaffold
Abstract
The invention relates to a preparation method of a three-dimensional porous antibacterial silk fibroin/graphene/titanium dioxide bone tissue engineering scaffold, comprising the following steps: 1) degumming silkworm cocoons and carrying out subsequent treatment to obtain a 1-4% aqueous solution of RSF; 2) ultrasonically dispersing a graphite oxide (GO) dispersion, titanium dioxide (TiO2) hydrosoland ethanol and blending, synthesizing rGO(at)TiO2 composite nanoparticles by a hydrothermal method, and washing and drying to obtain a powder; 3) ultrasonically dispersing the rGO(at)TiO2 powder inwater, and blending the rGO(at)TiO2 powder into the aqueous solution of RSF in 1); 4) concentrating the mixed solution in 3) until the mass percentage of RSF is 4-15%; 5) adding the aqueous solution of ethanol to the mixed solution of RSF/rGO(at)TiO2, carrying out mechanical stirring, and standing to form a gel; and 6) superfreezing the gel and freeze-drying to prepare the RSF/rGO(at)TiO2 three-dimensional porous tissue engineering scaffold. By using the rGO(at)TiO2 composite nanoparticles for synergetic enhancement of the RSF material, mechanical properties of the RSF scaffold are improved, and the scaffold also can be endowed with antibacterial property, osteoinductive capability and other functions.
Year: 2019
Country: CN
Doc No: 110354315