23 Jul Preparation method of submicron order core/shell structure PLGA (Poly(Lactic-co-Glycolic) microsphere with uniform particle size
Abstract
The invention discloses a preparation method of submicron order core/shell structure poly(lactic-co-glycolic) microsphere with a uniform particle size. The microsphere is of a core/shell structure, the particle size is 400-600nm, and the average shell thickness is 70nm. The preparation process comprises the following steps of: dissolving bovine serum albumin, carboxymethyl chitosan or low molecular weight heparin into a phosphate buffer liquid to be used as solution 1; dissolving PLGA and a emulsifier F127 into dichloromethane to be used as a solution 2; conducting ultrasonic dispersion on the solution 1 and the solution 2 so as to form a primary emulsion; further dropping the primary emulsion into a calcium chloride solution, and conducting ultrasonic dispersion in an ice bath so as to form a composite emulsion; and volatilizing the solvent, dialyzing, centrifugally washing, freezing and drying so as to obtain the PLGA microsphere. The preparation method has the advantages that the preparation process is simple, and meanwhile due to the core/shell structure of the microsphere, medicines can be wrapped into the core, the particle size is uniform, the medicine release action can be predicted, and the microsphere is convenient to be compounded with a tissue engineering bracket.
Year: 2013
Country: CN
Doc No: 103239408