23 Jul Preparation method of spongiocyte primary culture model for neurodevelopment research
Abstract
The invention relates to the technical field of gene engineering, and in particular, relates to a preparation method of a spongiocyte primary culture model for neurodevelopment research. The preparation method includes the concrete steps: a first step, taking a living creature, executing, sterilizing, taking out a brain tissue, and removing blood stains; a second step, transferring the whole brain treated in the first step into a culture dish, removing a brain membrane and blood vessels on the surface of the brain, flushing, and allowing the cerebral hemisphere to remain; a third step, snipping the brain tissue treated in the second step into pieces, grinding, adding 5 ml of 0.05% pncretin, transferring into a centrifuge tube, placing in a 37 DEG C cell incubator, and carrying out incubation digestion for 30 minutes; a fourth step, digesting until no obvious brain tissue mass exists, and terminating the digestion, to prepare a single cell suspension; a fifth step, centrifuging, and discarding the supernatant; and a sixth step, preparing a cell suspension, inoculating a culture plate with the cell suspension, and culturing at the temperature of 37 DEG C. The method is simple and easy to operate, and the success rate of spongiocyte culture is high.
Year: 2017
Country: CN
Doc No: 106609264