23 Jul Preparation method of round leaf zannichelliaceae cymodocea rotundta protoplast
Abstract
The invention discloses a preparation method of round leaf zannichelliaceae cymodocea rotundta protoplast, and belongs to the field of plant cell engineering. The preparation method comprises the stepS1 of taking spires of round leaf zannichelliaceae cymodocea rotundta, cleaning the spires with sterile seawater, and then performing temporary culture to obtain a raw material 1; the step S2 of immersing the raw material 1 obtained in the step S1 into a pretreatment solution to obtain a raw material 2; the step S3 of taking out the raw material 2 obtained in the step S2, removing the moisture, cutting the raw material into pieces and adding an enzyme solution for enzymatic hydrolysis to obtain an enzymatic hydrolysis tissue; the step S4 of filtering the enzymatic hydrolysis tissue obtained in the step S3 to obtain a filtrate; the step S5 of centrifuging the filtrate obtained in the step S4, abandoning a supernatant, conducting suspension and precipitation with a W5 solution, centrifugingand abandoning the supernatant to obtain a precipitate; the step S6 of suspending the precipitate obtained in the step S5 in an MMG solution to obtain the round leaf zannichelliaceae cymodocea rotundta protoplast. The round leaf zannichelliaceae cymodocea rotundta protoplast obtained by the method has high yield and high survival rate.
Year: 2019
Country: CN
Doc No: 109880787