23 Jul Preparation method of pyrus betulaefolia protoplast
Abstract
The invention belongs to the technical fields of cytobiology and biology, and discloses a preparation method of pyrus betulaefolia protoplast. According to the method, through the pretreatment of a pyrus betulaefolia material and separation treatment of cytoplasm and cytoderm, the mesophyll tissue of pyrus betulaefolia is subjected to enzymolysis through the combination of cellulase, macerase and pectinase, the preparation of pyrus betulaefolia protoplast is effectively realized, the problem that the pyrus betulaefolia protoplast is difficult to separate is overcome, and the preparation method of the pyrus betulaefolia protoplast is effectively created. The method breaks through the bottleneck of genetic transformation of pyrus betulaefolia, establishes a pyrus betulaefolia cell engineering technical system, and provides a technical base for the genetic transformation of the pyrus betulaefolia protoplast, the transient expression examination of gene and the fusion of the protoplast.
Year: 2017
Country: CN
Doc No: 106967670