23 Jul Preparation method of nano-fiber scaffold for promoting cartilage regeneration
Abstract
The invention belongs to the field of tissue engineering, and discloses a preparation method of a nano-fiber scaffold for promoting cartilage regeneration to solve the problem that Nell-1 serving as anovel cartilage-derived growth factor can promote cartilage bone cell functions, but is easily inactivated in the process of being integrated into a tissue engineering cartilage scaffold. The preparation method includes the steps: firstly, preparing Nell-1 supported chitosan nano-particle solution and collagen/poly (L-lactic acid)-copolymer (epsilon-caprolactone) mixed solution; secondly, preparing a PLLA-CL/collagen nano-fiber mesh by coaxial electro-spinning and dynamic liquid collection technology; finally, preparing the collagen/HA mixed nano-fiber scaffold by collagen and hyaluronic acid(HA). The operation method is simple, the prepared nano-fiber scaffold is good in biological compatibility, biological activity of the growth factor can be protected, the release time of the growth factor is prolonged, and cartilage repair and regeneration can be promoted.
Year: 2018
Country: CN
Doc No: 107802888