23 Jul Preparation method of mulberry silk tissue engineering scaffold
Abstract
The invention relates to a preparation method of a mulberry silk tissue engineering scaffold. Artificially synthesized macromolecular material PLGA (polylactic-co-glycolic acid) is adopted in the traditional method, degradation products of the PLGA are acid, local pH value is reduced and inflammation can be easily caused. The preparation method provided by the invention comprises that mulberry silk is taken as a raw material and is dissolved with CaCl2 solution with the mass fraction of 40% after being refined by Na2CO3 solution with the concentration of 0.5g/dL, then filtering is carried out by adopting a dialysis bag, the obtained fibroin protein solution is added into mixed solution of chitosan/acetic acid, stirring to be uniform is carried out, then mixture is added into a charging barrel of a sprayer of forming equipment, low-temperature forming is carried out, a freezing scaffold is placed into a freeze drying machine, freeze drying is carried out, and a scaffold which is in solid state at normal temperature is obtained. The degradation products of the mulberry silk tissue engineering scaffold prepared by the preparation method provided by the invention are polypeptides, local pH value can not be reduced, and inflammation can not be caused, thus the mulberry silk tissue engineering scaffold is harmless to human body.
Year: 2012
Country: CN
Doc No: 102600504