23 Jul Preparation method of human TDP-43 monoclonal antibody coating ELISA plate and ELISA detection kit
Abstract
The invention relates to a preparation method of a new human TDP-43 monoclonal antibody coating ELISA plate and ELISA detection kit. The key technology mainly lies in that: genetic engineering method is utilized to prepare a specific mouse anti-human monoclonal antibody, and the ELISA plate by which the antibody is purified and then coated is taken as important component of the kit, and then the ELISA plate, TDP-43 polyclonal antibody and horseradish peroxidase labelled second antibody, TDP-43 standard substance, TDP-43 positive control, sample diluent, cleaning solution, development solution and stop solution are assembled into a humanized TDP-43 ELISA detection kit; a sensitive and rapid method for detecting concentration of TDP-43 in ELISA antibody capturing human serum or cell and tissue culture is established, and the method has high specificity and high stability. The kit is mainly used in general libratory or neuropathology clinical research.
Year: 2010
Country: CN
Doc No: 101634656