23 Jul Preparation method of heparin and twin factor synergistically regulated P(LLA-CL)/collagen bilayer intravascular stent
Abstract
The invention relates to a preparation method of a heparin and twin factor synergistically regulated P(LLA-CL)/collagen bilayer intravascular stent. The method comprises the following steps: uniformly mixing P(LLA-CL) with collagen in a solvent to obtain a composite spinning solution; dissolving heparin sodium and VEGF in a dilution solution to obtain an internal layer supported medicine solution; dissolving PDGF in the dilution solution to obtain an external layer supported medicine solution; carrying out coaxial electrostatic spinning with the internal layer supported medicine solution as a core layer and the spinning solution as a shell layer to obtain a intravascular stent internal layer; and carrying out bidirectional conjugate electrostatic spinning with the external layer loaded medicine solution as a core layer and the spinning solution as a shell layer to obtain an intravascular stent external layer, continuously receiving the intravascular stent external layer at the outer side of the intravascular stent internal layer to obtain a bilayer intravascular stent, and cross-linking to obtain the heparin and twin factor synergistically regulated P(LLA-CL)/collagen bilayer intravascular stent. The intravascular stent provided by the invention has excellent mechanical performances and biocompatibility, has natural blood vessel simulating components, structure and functions, is in favor of realizing in situ regeneration of blood vessel tissues and reconstruction of a multilayer structure, and has important applications in the blood vessel tissue engineering.
Year: 2016
Country: CN
Doc No: 105233339