23 Jul Preparation method of hard tissue engineering scaffold with gene regulation function
Abstract
The invention discloses a preparation method of a hard tissue engineering scaffold with a gene regulation function. The preparation method comprises the steps of adopting a method combining a sol-gel technology and an electro-spinning technology for preparing CaO-P2O5-SiO2 system mesoporous bioactive glass micro-nanofiber as an inorganic base phase; utilizing a gene recombination technology for preparing plasmid DNA (Deoxyribonucleic Acid) loaded with an osteocyte regulatory factor Osterix target gene, and using chitosan for embedding the plasmid DNA to prepare into a microballoon as an additive phase; preparing an emulsion system formed by the bioactive glass micro-nanofiber, the genetic vector microballoon, PCL and other materials, utilizing a thermally induced phase separation method for preparing a bioactive glass/PCL bionicbone tissue engineering scaffold compounding the Osterix plasmid DNA, and obtaining the hard tissue engineering scaffold with the gene regulation function. The hard tissue engineering scaffold can stimulate cells on a molecular level and produces a special response so as to realize accurate regulation on cell growth genes.
Year: 2017
Country: CN
Doc No: 106943624