23 Jul Preparation method of functional tissue engineering bracket based on collagen and bacterial cellulose
Abstract
The invention provides a preparation method of a functional tissue engineering bracket based on collagen and bacterial cellulose. The preparation method comprises the following steps: adding the bacterial cellulose into imidazoles ionic liquid to obtain a bacterial cellulose solution; adding a sodium periodate solution into the bacterial cellulose solution to obtain a DABC solution; dissolving the collagen into the DABC solution to obtain a CDABC solution; adding template particles into the CDABC solution to obtain a dispersed solution; pouring the dispersed solution and a surfactant into an organic solvent, and homogenizing and emulsifying to obtain w/o type CDABC emulsion; adding a precipitant into the CDABC emulsion and collecting sediment; after washing and extracting the sediment, freezing and drying to obtain a CDABC porous bracket; mixing a medical protein solution with the CDABC porous bracket; carrying out vacuum vibration and adsorption; centrifuging and collecting sediment; washing, freezing and drying the sediment to prepare the functional CDABC porous bracket. A reaction product provided by the invention has good stability performance and high reaction efficiency. By loading growth factors, the bracket can be used for effectively facilitating cell reproduction and tissue regeneration, and has higher functionality.
Year: 2016
Country: CN
Doc No: 105641746