23 Jul Preparation method of cartilage cell primer layer, and cartilage tissue
Abstract
The invention relates to the field of cartilage tissue engineering, in particular to a preparation method of a cartilage cell primer layer, and cartilage tissue. The preparation method of the cartilage cell primer layer comprises the steps that a carrier and autologous cartilage cells are subjected to three-dimensional dynamic co-culture, and then the cartilage cell primer layer is obtained; according to the culture conditions of the three-dimensional dynamic co-culture, the temperature is 36.5-37.5 DEG C, and 4.5-5.5% of CO2 and 9-11% of O2 exist; a culture system of the three-dimensional dynamic co-culture internally comprises 9-12 ng/mL of basic fibroblast growth factors; before the three-dimensional dynamic co-culture, the density of the primary autologous cartilage cells in the carrier is (1.6-2.0)*10<5>/cm<2>; the carrier comprises bone cement; and the composition of the culture system of the three-dimensional dynamic co-culture comprises a DMEM culture medium, 4-5mM L-glutamyl,serum with the volume fraction being 9-11% and double antibiotics. The prepared cartilage cell primer layer is advantageously fused with the autologous cartilage cells and has good biocompatibility.
Year: 2019
Country: CN
Doc No: 109251887