23 Jul Preparation method of bio-functionalized chitosan hydrogel
Abstract
The invention belongs to the field of tissue engineering biological materials, and specifically relates to a preparation method of bio-functionalized chitosan hydrogel. The preparation method comprises the following steps: preparing genipin solutions with different weight percentages: (1) individually dissolving 0.005 gram of genipin, 0.01 gram of genipin, and 0.02 gram of genipin into 1 mL of alcohol (75%) to prepare a genipin solution (0.5%), a genipin solution (1.0 %), and a genipin solution (2.0%); (2) preparing a chitosan solution: dissolving 1.5 grams of chitosan into 100 mL of acetic acid solution (3%) to prepare a chitosan solution (1.5%), adding biological molecules containing amino groups, and stirring the mixture in a bottle for a whole night; (3) mixing two solutions according to different ratios, and allowing the mixed solution to stand still for 24 to 72 hours to obtain the functionalized chitosan hydrogel; wherein the mole ratio of genipin to biological molecules containing amino groups is maintained in a range of 10:5 to 10:1; and the mole ratio of chitosan to genipin is in a range of 300:1 to 30:10. The operation is simple, the cost is low, the reaction conditions are mild and matured, the cytotoxicity is little or none, and the method is universal.
Year: 2016
Country: CN
Doc No: 105920675