23 Jul Preparation method of bacterial cellulose reinforced amnion composite material used for corneal reconstruction
Abstract
The invention discloses a preparation method of a bacterial cellulose reinforced amnion composite material used for corneal reconstruction, and relates to a preparation technology of a tissue engineering biomaterial. The preparation method comprises the following steps: selecting strains secreting bacterial celluloses, activating to prepare a seed mash, and allowing the seed mash with the concentration of strains of 30-50wt% to stand in a culture medium for culturing to obtain a bacterial cellulose membrane; immersing a cell-free disinfected dry amnion in the culture medium, and tiling the amnion on the surface of the bacterial cellulose membrane after the amnion is completely wet; and allowing the seed mash to continuously stand for culturing for 1-3d, purifying, and drying to obtain the bacterial cellulose reinforced amnion composite material used for corneal reconstruction. The preparation method has the advantages of simplicity, easy implementation, low cost, wide material source, and the obtained composite material has the advantages of good spatial three-dimensional net structure, high transparency, good biocompatibility and high mechanical strength, and can be used for restoring all corneal damages as a tissue engineering cornea reconstruction support material.
Year: 2014
Country: CN
Doc No: 103816571