Preparation method of artificial liver cell microfluidic microcapsules and microfluidic microcapsule generator of preparation method

Preparation method of artificial liver cell microfluidic microcapsules and microfluidic microcapsule generator of preparation method

Abstract

The invention relates to the technical field of tissue engineering and laboratory equipment, in particular relates to a preparation method of artificial liver cell microfluidic microcapsules and special equipment of the method, a microfluidic microcapsule generator. According to the invention, by utilizing a chemical principle that sodium alginate reacts with calcium chloride to generate a calcium alginate hydrogel, through the design of a microfluidic device, microcapsules stable in size are formed by virtue of the effects such as shear force, surface tension and the like of continuous phase liquid to dispersed phase liquid. According to the artificial liver cell microcapsules prepared by the invention, the function of seed cells in the microcapsules can be maintained for a long time, hazardous substances can be still metabolized under in vitro liver failure similar to the plasma influence and an effect of an inflammatory factor in a body, a certain liver function is maintained, and the artificial liver cell microfluidic microcapsules are hopefully applied to a bioartificial liver system and cell therapy.

Year:  2016

Country:  CN

Doc No:  105457100

 

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