23 Jul Preparation method of accellular amniotic membrane for skin biological stents of tissue engineering
Abstract
The invention relates to the technical field of medical materials, and discloses a preparation method of an accellular amniotic membrane for skin biological stents of tissue engineering. The preparation method comprises the following steps of rinsing and disinfecting the amniotic membrane, soaking into DMEM (dulbecco’s modified eagle medium) and glycerin, and storing for 6 months at the temperature of -80 DEG C; repeatedly freezing and thawing the soaked amniotic membrane for 2 to 4 times at the temperature of -80 to 37 DEG C, removing the DMEM and the glycerin, and hatching in trypsin-EDTA in an overnight way; terminating digestion of the hatched amniotic membrane by DMEM containing serum, using PBS (poly(butylene succinate)) to flush, and freeze-drying, so as to obtain the accellular amniotic membrane. The preparation method has the advantages that the repeated freezing and thawing type physical accellular method is combined with the trypsinization type chemical accellular method, and the technology steps are adjusted to be adapted, so that the cells in the amniotic membrane are promoted to be thoroughly removed at high efficiency, the mechanical damage is avoided, and more cell factors in the amniotic membrane are maintained.
Year: 2017
Country: CN
Doc No: 107233623