23 Jul Preparation method for wharton jelly tissue engineering scaffold of human umbilical cord
Abstract
The invention discloses a preparation method for a wharton jelly tissue engineering scaffold of human umbilical cord. The preparation method specifically comprises the following steps: S1) separatingwharton jelly; removing blood vessels from fresh umbilical cord and then cutting into pieces; S2) mechanically smashing: adding wharton jelly cut in step S1 into normal saline, and then uniformly shaking into slurry. The invention relates to the technical field of biomedicine. The preparation method for the wharton jelly tissue engineering scaffold of human umbilical cord is capable of preparing the wharton jelly tissue engineering scaffold of human umbilical cord without cells by adopting cell-removing, freeze-drying and cross-linking processes; ingredients, such as glycosaminoglycan and II type collagen, in wharton jelly are remained in the prepared tissue engineering scaffold; cells inoculated onto the scaffold are adhered to scaffold duct walls and are under excellent growth state; substances are secreted; an in vivo test proves that no immunoreaction exists; the product has a bright application prospect, so that mechanical properties of scaffold are greatly promoted, antigenicityis effectively eliminated and a controllable degradation rate is achieved.
Year: 2019
Country: CN
Doc No: 109675114