23 Jul Preparation method for tissue engineering nerves of compound seed cells
Abstract
The invention discloses a preparation method for tissue engineering nerves of mesenchymal stem cells induced by compound nerves. The preparation method for tissue engineering nerves of mesenchymal stem cells induced by compound nerves comprises the steps of extracting nerves with an improved enzyme-hypotension-chemical detergent in a trigeminy manner based on a Sondell preparation method, constructing tissue engineering peripheral nerves with biological activity in vitro, and then compounding mesenchymal stem cells which are subjected to in-vitro cultivation, amplification and neuronal differentiation induction. According to the preparation method provided by the invention, the destructive effect caused by an existing acceptable chemical preparation method, namely the Sondell method, on a nerve basal membrane tube and an extracellular matrix structure to a certain extent is overcome; the influence on axonal regeneration is alleviated. The tissue engineering nerves of the mesenchymal stem cells induced by the compound nerves, which are prepared by the method disclosed by the invention, can be applied to the preparation of nerve grafts and have good effects in restoring peripheral nerve defect.
Year: 2013
Country: CN
Doc No: 103446628