23 Jul Preparation method for decellularized tissue membrane
Abstract
The invention relates to a preparation method for a decellularized tissue membrane. The method comprises the following steps: 1) removing adipose tissue on the tissue membrane, washing and cleaning bloodstain; 2) placing the neutralized and washed tissue in surfactant solution and soaking, and adequately removing impure proteins; 3) placing in protein renaturation solution and soaking; 4) placing in an organic solvent and soaking, and separating out grease; 5) placing in alkaline solution and soaking, neutralizing, and preparing pores on the tissue membrane; 6) placing the tissue membrane in solution containing a crosslinking agent and soaking, crosslinking; and 7) executing the radiation sterilization to the tissue membrane obtained in the steps in the wet state; or executing the radiation sterilization or EO sterilization in the freeze-drying mode. The method is capable of removing antigens, such as cells, lipids and impure proteins, in the human source or animal source tissue membrane, keeping and extracellular matrix and generating the suitable pores. The prepared decellularized tissue membrane can be used as a tissue engineering bent, a patching material for tissue membrane damage and a wound surface dressing.
Year: 2017
Country: CN
Doc No: 107233622