23 Jul Preparation method for antibacterial collagen
Abstract
The invention relates to a preparation method for antibacterial collagen. The method comprises the following steps: (1) dissolving collagen in a buffer solution and controlling the mass concentration of collagen to be 0.1%-15%, thereby obtaining a collagen solution; (2) adding modified triclosan into the collagen solution obtained in the step (1), increasing the temperature to 25-50 DEG C and reacting for 0.1-48h, thereby obtaining an antibacterial collagen solution; (3) dialyzing the antibacterial collagen solution obtained in the step (2) for 0.5-96h at 0-4 DEG C, and then freeze-drying, thereby obtaining the antibacterial collagen. The antibacterial collagen prepared according to the method has the advantages that the antibacterial components are firmly combined with collagen through covalent bonds; the antibacterial effect is long-lasting; if the antibacterial collagen is prepared into the biomedical materials, including antibacterial collagen gel, collagen sponge, wound dressing, cytoskeleton of tissue engineering, and the like, the material has an antibacterial effect in the whole use period.
Year: 2017
Country: CN
Doc No: 106349718