23 Jul Preparation method and use of graft material in double membrane structure
Abstract
The invention belongs to the field of tissue engineering and biomaterials and discloses a preparation method and use of a graft material in a double membrane structure. The graft material comprises stem cell membrane patch segments and platelet-rich fibrin membrane particles, wherein the stem cells are firstly expanded-cultured, and then induction-cultured by using a membrane induction fluid to obtain a stem cell membrane patch, and the stem cell membrane patch is sheared to prepare the cell membrane patch segment; a platelet-rich fibrin gel is extruded, then liquid content of the extruded platelet-rich fibrin gel is removed to obtain the platelet-rich fibrin membrane, and the platelet-rich fibrin membrane is sheared to form particles; and the cell membrane patch segments and the platelet-rich fibrin membrane particles are mixed according to an optimal proportioning relation of in-vitro screening to prepare the needed graft material. The graft material can be widely applied to tissue repair and regeneration of small-area lesion in oral activity and other parts of the body, and the tissue repair effect is improved.
Year: 2013
Country: CN
Doc No: 103100112