23 Jul Preparation method and application of tissue-engineering human tooth root implantation material
Abstract
The invention relates to the fields of oral medicine and biomedicine, particularly a preparation method and application of a tissue-engineering human tooth root implantation material. The preparation method of the tissue-engineering human tooth root implantation material comprises the following steps: (1) separating, culturing and identifying human root tip dental papilla stem cells; (2) constructing an NFIC lentivirus vector; (3) preparing NFIC lentivirus vector transfected root tip dental papilla stem cells, screening the overexpression NFIC cell line, and inducing the cell line in a mineralization mode; and (4) incubating the overexpression NFIC cell line subjected to mineralization induction and a hydroxyapatite/tricalcium phosphate stent material together. The preparation method is simple to operate; and the prepared tooth root implantation material has small immunoreaction, and can be applied to preparation of human tooth roots.
Year: 2012
Country: CN
Doc No: 102755667