23 Jul Preparation method and application of sulfhydryl/disulfide bond controllable self-crosslinked hyaluronic acid hydrogel
Abstract
The invention discloses a preparation method and an application of sulfhydryl/disulfide bond controllable self-crosslinked hyaluronic acid hydrogel and belongs to the field of biological materials. The hydrogel is prepared from a sulfhydryl-hyaluronic acid compound with free sulfhydryl as a raw material; by adjusting the sulfhydryl density (10-60%), the gel temperature (4-37 DEG C) and the molecular weight (0.1M, 0.3M and 1M) of hyaluronic acid, and using the oxidation-reduction transformation characteristics between sulfhydryl and disulfide bonds to control the forming time, degradation time and the mechanical properties of the gel, a controllable and injectable type intelligent hydrogel with a good three-dimensional network crosslinked structure is constructed. Meanwhile, the hydrogel of the type is single in component, avoids the introduction of exogenous toxic substances before and after gel forming, and has good biocompatibility and degradation performance. Based on the research discovery, the controllable self-crosslinked high-polymer polymer can be applied to minimally invasive repairing of tissue engineering in-situ damages and construction of an intelligent adjustable three-dimensional cell culture scaffold.
Year: 2015
Country: CN
Doc No: 104892962