23 Jul Preparation method and application of silk fibroin and chitin blended nanofiber embedded hydrogel cartilage biomimetic scaffold
Abstract
The invention discloses a preparation method and application of a silk fibroin and chitin blended nanofiber embedded hydrogel cartilage biomimetic scaffold. The method utilizes mulberry silk to prepare regenerated the silk fibroin, purifies industrial grade chitin, mixes a silk fibroin solution and a chitin solution, prepares a blended nanofiber membrane by an electrospinning technique, cross-links with ethanol to form a short fiber through a blended nanofiber, mixes the short fiber and the silk fibroin solution, conducts cross-linking by a crosslinking agent, and conducts freeze-forming to obtain a spongy cartilage bionic scaffold. In particular, the insertion of the nano short fiber increases the compressive strength and biocompatibility of the scaffold material. The method constructs the biomimetic scaffold with good biocompatibility, low immune rejection and similar composition with natural cartilage matrix, has wide material source, low cost and simple preparation process, can beused for constructing tissue engineering cartilage and repairing cartilage regression, and has good clinical application prospects.
Year: 2019
Country: CN
Doc No: 109999227