23 Jul Preparation method and application of silk bracket, and three-phase silk ligament graft and preparation method thereof
Abstract
The invention discloses a preparation method and application of a silk bracket, and a three-phase silk ligament graft and a preparation method thereof. Silks are woven into a net-shaped bracket with a macroporous structure; the bracket is divided into three areas: a ligament area, a cartilage area and a bone area; mesenchymal stem cells, mesenchymal stem cells carrying with TGF-beta gene lentiviral transfection and mesenchymal stem cells carrying with BMP-2 gene lentiviral transfection are respectively planted in the three areas; the net-shaped bracket modified by the divided areas is rolled to form a bionic ligament graft with a physiological transition structure. The method avoids the problem of stress concentration due to direct connection of the soft and hard tissues; the planting gene-modified mesenchymal stem cells have excellent directional differentiation features, so that the cultivating time and steps of planting a plurality of different cells are saved, and the problem of weak biological fixation of the ligament graft-bone combination part in the existing single-phase tissue engineering ligament and the problem of the traditional method incapable of enabling the cells to grow deeply are solved.
Year: 2014
Country: CN
Doc No: 103505761