23 Jul Preparation method and application of pancreas substrate hydrogel for repairing pancreatic tissue
Abstract
The invention belongs to the technical field of tissue engineering and regenerative medicines, aims to solve the technical problems that in the prior art pancreas functions are hard to recover and pancreas tissue injury caused by acute necrotizing pancreatitis is hard to recover, and provides a preparation method and application of pancreas substrate hydrogel for repairing pancreatic tissue. The preparation method comprises the following steps: B, preparing pancreas substrate hydrogel, namely performing freeze-drying on decellularized pancreatic tissue, powdering, mixing with a hydrochloric acid-stomach protease solution according to a ratio, and adjusting the pH value to a physiological status, thereby obtaining the pancreas substrate hydrogel. The pancreas substrate hydrogel provided by the invention not only has the advantages of temperature sensitivity, low immunogenicity and capability of providing extracellular microenvironments and the like, but also is simple in operation process, gentle in implementation condition and capable of providing a hydrogel material to pancreas tissue engineering. The material is applicable to growth of pancreas cells, anti-inflammatory M2 type polarization of macrophage can be regulated and controlled, and endogenous repairing of severe pancreatitis pancreatic tissue is promoted.
Year: 2017
Country: CN
Doc No: 107158463