23 Jul Preparation method and application of mouse MIP3alpha-endoglin Fc fusion protein
Abstract
The invention belongs to the technical field of genetic engineering and discloses a preparation method and application of mouse MIP3alpha-endoglin Fc fusion protein. The preparation method includes: taking mMIP3alpha-F, R1 as a primer expansion template mouse MIP3alpha plasmid; taking F1, mEndoglin-R as a primer amplification template mouse Endoglin; taking a first-round PCR product as a template, and taking mMIP3alpha-F and mEndoglin-R as primers for PCR amplification; pcDNA3.1 (+)/Fc plasmid and a target fragment use AflII/BamHI digestion and recycling for connecting, converting, plate coating and screening. The preparation method and the application are about to be very likely to greatly enhance tumor tissue targeting performance of the fusion protein and obviously improve antitumor immunoactivity of the fusion protein, and the fusion protein shows great clinical application value.
Year: 2019
Country: CN
Doc No: 110257414