23 Jul Preparation method and application of deproteinated decalcification bone matrix implantable microcarrier
Abstract
The invention provides a preparation method of a deproteinated decalcification bone matrix implantable microcarrier and an application of the deproteinated decalcification bone matrix implantable microcarrier in preparation of tissue engineering materials. The preparation method comprises the following steps: carrying out cryogenic grinding on bovine cortex bones, sifting out bone particles with particle sizes of less than 200 micrometers, and carrying out degreasing, decalcification and deproteinization treatment on the bone particles. The deproteinated decalcification bone matrix implantable microcarrier obtained by using the preparation method is low in immunogenicity and high in cell adhesion rate. Cells cultivated by adopting the deproteinated decalcification bone matrix implantable microcarrier under a static cultivation condition are high in activity, stem cell phenotypes are kept perfectly, and as protein active ingredients in a decalcification bone matrix are removed, the deproteinated decalcification bone matrix particles do not cause change of bone components in target cells such as stem cells; and dynamic cultivation experiments of BMSCs (bone marrow mesenchymal stem cells) prove that the microcarrier particles are available for dynamic suspension cultivation conditions and can successfully form suspension at a stirring rate suitable for cell cultivation, compared with the cell adhesion rate of cytodex3, the cell adhesion rate of the microcarrier particles is higher, and the amplification efficiency of the microcarrier particles is similar to that of the cytodex3.
Year: 2014
Country: CN
Doc No: 103990180