23 Jul Preparation cryopreservation method and application of human placental subchorionic villi aorta tissue
Abstract
The invention discloses a preparation cryopreservation method of the human placental subchorionic villi aorta tissue. The method comprises the steps that (1) a placenta is washed cleanly; the abscisicdecidua is cut off along the edge of the placenta, and the amniotic membrane is removed; (2) the placenta fetal surface chorion with the amniotic membrane and the aortas are separated and washed withnormal saline or PBS buffer liquid, the blood vessels are washed so as to remove the stranded blood clots in the blood vessels; and then, the aortas connected with the chorion lamina membranacea is cut off one by one; and (3) the blood vessels are transferred into a cryopreserved pipe or a cryopreserved bag, vitrified cryopreservation liquid is guided through a three-step method, the blood vessels are transferred into a programmed freezer so as to be cooled at -80 – -90 DEG C, and then the blood vessels are transferred to liquid nitrogen cryopreservation. The cryopreservation method is beneficial for improvement of the activity of the cryopreserved tissue and the cells, the morphology, the function and the structure are consistent with the fresh tissue after being recovered, the preservedtissue can not only be applied to fields of separation of stem cells and epithelial cells, but also can be applied to fields of tissue engineering transplantation.
Year: 2018
Country: CN
Doc No: 109042624