23 Jul Preparation and cryopreservation method and application of human placental chorionic tissues
Abstract
The invention discloses a preparation and cryopreservation method of human placental chorionic tissues. The method comprises the following steps: (1) cleaning a placenta, and cutting off a shed decidua along the edge of the placenta to remove the amnion; and (2) separating the amnion-removed placenta fetus chorion and large vessels to obtain remaining placenta tissues which are placental chorionictissues, cutting the placental chorionic tissues to form small blocks, cutting the small blocks to form slices, placing the slices in a cryopreservation bag or a cryopreservation tube, guiding a vitrification cryopreservation solution through a three-step process, transferring the obtained mixture into a programmable cooler, reducing the pressure to -80 to -90 DEG C, transferring the cooled mixture to liquid nitrogen, and performing cryopreservation. The cryopreservation method facilitates the improvement of the activity of cryopreserved tissues and cells, the morphology, the function and thestructure of resuscitated cryopreserved tissues are same to those of fresh tissues, the overall survival rate of cells in the tissues reaches 90% or above, and the preserved tissues can be used in the field of separation of stem cells and epithelial cells, and also can be used in the field of tissue engineering transplantation.
Year: 2018
Country: CN
Doc No: 108812641