23 Jul Preparation and applications of heavy metal super-enriched transgenic engineering rice adopting transgenic sedum plumbizincicola SpHMA3 gene
Abstract
The invention discloses preparation of heavy metal super-enriched transgenic engineering rice adopting the transgenic sedum plumbizincicola SpHMA3 gene. The preparation comprises the following steps:cloning the SpHMA3 gene in sedum plumbizincicola; based on a plant expression vector, constructing a transformation vector containing a strong promoter and a screening gene, and inserting the SpHMA3 gene into the transformation vector through a homologous recombination method, thus obtaining an excessively expressed binary vector; transferring the excessively expressed binary vector into the ricecallus tissue through agrobacterium tumefaciens, culturing transgenic seedlings, and screening transgenic positive plants, wherein the transgenic receptor plants belong to the rice Nipponbare variety.With the cultivating method provided by the invention, the problems that for the wild heavy metal enriched plants, the tolerance is limited, the growth is slow, the biomass is small, the growing environment is special, heavy metal returns, etc., are solved. In addition, the invention further provides applications of the heavy metal super-enriched transgenic plant obtained through the preparationof the heavy metal super-enriched transgenic engineering rice adopting the transgenic sedum plumbizincicola SpHMA3 gene in heavy metal repairing of soil.
Year: 2018
Country: CN
Doc No: 108384802