23 Jul Preparation and application of high expression TSP-1 stem cell for promoting regeneration of neuron axon
Abstract
The invention relates to the technical field of neurological rehabilitation tissue engineering and specifically discloses a preparation method of a TSP-1 gene modified mesenchymal stem cell. The preparation method comprises the following steps: 1, constructing a TSP-1-carrying adenovirus vector: preparing a pHBAd-MCMV-GFP vector and a TSP-1 target fragment with a double digestion method, connecting a target gene and the pHBAd-MCMV-GFP vector, and identifying pHBAd-MCMV-GFP-TSP-1 with a sequencing method, so that the correctness of an exogenous gene sequence is ensured; 2, transfecting BMSCs with a TSP-1 adenovirus, so as to obtain the TSP-1 gene modified mesenchymal stem cell. In the preparation method, by selecting the TSP-1 gene modified mesenchymal stem cell, the recovery of transplanted neurons of the neurological rehabilitation tissue engineering and the regeneration of neurite can be accelerated, and the construction speed of a damaged local neuronal circuit is accelerated.
Year: 2018
Country: CN
Doc No: 107858374