23 Jul Porcine adipose tissue-specific chimeric promoters
Abstract
The invention belongs to the technical field of animal genetic engineering, and specifically relates to cloning, functional verification and application of two porcine adipose tissue-specific chimeric promoters. According to the invention, the activity of an adi-pro regulating element is verified, and different chimeric promoters containing a pCMVIE promoter and an SV40 enhancer are constructed through a chimeric method, so that the activity of the adi-pro regulating element is greatly improved and new specific expression promoter resources are provided for genetic engineering and molecular breeding. The nucleotide sequences of the promoters are respectively as shown in the sequence tables SEQ ID NO: 4 (sequence identity number: 4) and SEQ ID NO: 5.
Year: 2012
Country: CN
Doc No: 102604947