23 Jul Pluripotent stem cells MDPSCs for dental pulp regeneration, and isolated culture method and application thereof
Abstract
The invention provides pluripotent stem cells MDPSCs for dental pulp regeneration, and belongs to the technical field of tissue engineering. The MDPSCs are CD24a positive cells isolated from a dentalmesenchymal tissue. The MDPSCs show a self-renewal ability under 3D culture conditions, and cell spheres with positive expression of pluripotent related marker genes Sox2, Nanog, Oct4 and Ki67 are formed. The invention also provides a preparation method and an application of the pluripotent stem cells. The pluripotent stem cells MDPSCs are successfully separated and enriched by a 3D culture method, and the characteristic surface markers of the MDPSCs are defined by combining with flow cytometry. The transplantation of the MDPSCs in nude mice can regenerate dental pulp, the traditional root canal therapy is replaced, and the vital pulp is preserved on the premise of eliminating pulpitis. The pluripotent stem cells MDPSCs have the ability to form a vascularized dental pulp-dentin complex, and provide a cellular basis for study of functional dental pulp regeneration and clinical transformation application.
Year: 2019
Country: CN
Doc No: 110408592