23 Jul Plant transgenic selection system using betaine aldehyde dehydrogenase (BADH) as marker gene and use thereof
Abstract
The invention discloses a plant transgenic selection system using betaine aldehyde dehydrogenase (BADH) as a marker gene and use thereof and belongs to the technical field of gene engineering. In the system, BADH is used as a marker gene of an exogenous gene and NaCl is used as a selecting agent. The use comprises the following steps: (1) determining NaCl concentration which can just inhibit plant tissue differentiation; (2) transferring the exogenous gene which contains the BADH marker gene into a plant cell or tissue; and (3) culturing the transgenic tissue or cell into which the BADH marker gene is transferred on a culture medium in which the NaCl concentration determined by the step (1), obtaining the transgenic tissue or cell into which the BADH marker gene is transferred into if the transgenic tissue or cell can grow, and confirming by molecular detection. The invention has the advantages of greatly lowering the cost investment in a transgenic test, solving the safety problem of transgenic organisms, and providing low cost selection for the construction of a safe and effective selection system for plant genetic transformation.
Year: 2012
Country: CN
Doc No: 102304506