23 Jul Plant gene editing method independent on genotype
Abstract
The invention discloses a plant gene editing method independent on genotype, relates to the field of plant genetic engineering and solves the problems that descendant transgenic ingredients are removed unfavorably as a result of great heritable variation because existing gene edition is limited by genotype and tissue culture is needed. The method comprises the following steps: preparing an intermediate carrier with a tool attribute for gene edition by taking a transgenic plant gene editing target point gRNA and a gene editing system element and a plant strain with haploid inducting efficiencyas materials; inducting a plant to generate haploids by taking the intermediate carrier as a male parent and finishing gene edition at the same time; and carrying out natural or manual doubling to finally obtain a plant new variety/strain homozygote of the targeted gene edition. The method is free of complex genetic transformation and tissue culture processes after preparing the gene editing intermediate carrier tool, and achieves gene edition of different genotypes of a same plant by means of manual cross pollination.
Year: 2019
Country: CN
Doc No: 109182371