23 Jul PK-15 cell line for expressing Nectin-1 gene extracellular region segment and building method thereof
Abstract
The invention belongs to the technical field of gene engineering, in particular relates to building of a PK-15 cell line PK15-nectin-1-ecto capable of expressing a Nectin-1 gene extracellular region segment. A Nectin-1 extracellular region complete encoding region sequence in a pig liver tissue is cloned onto a slow virus core carrier pLenti-7.3CA plasmid, a pLenti7.3CA-nectin-1-ecto recombinant plasmid is successfully built, the recombinant plasmid and another three helper plasmids, namely pLP1, pLP2 and pLP/VSVG are jointly transfected to a packaging cell, namely a human embryo kidney cell 293FT, so as to obtain an HIV (Human Immunodeficiency Virus) slow virus particle, and the slow virus particle is transduced to a PK-15 cell by adopting a low-speed centrifugal method, so as to obtain a PK-15 cell line PK15-nectin-1-ecto capable of stably expressing pig pLenti7.3CA-nectin-1-ecto. The PK15-nectin-1-ecto cell line contains the Nectin-1 gene extracellular region segment, and the cell line obtains anti-pseudorabies virus capability.
Year: 2012
Country: CN
Doc No: 102329776