23 Jul Pig muscular tissue specificity induced expression system
Abstract
The invention belongs to the technical field of animal genetic engineering, and particularly relates to construction of a muscular tissue specificity expression system. The muscular tissue specificity expression system is constructed by a core promoter of a pig skeletal muscle specificity expressed gene alpha-actin and an ecdysone induced expression system and can be used for realizing specific expression of target genes in muscles and accurate expression of the target genes. The muscular tissue specificity expression system is characterized in that an adjusting plasmid in the ecdysone induced expression system is replaced by the core promoter zone of the pig skeletal muscle specificity expressed gene, a beta-galactosidase reporter gene is inserted in multiple cloning sites of a reaction substance of the ecdysone induced system, and two recombinant vectors are used as issue specificity induced expression systems and are co-transfected to rat C2C12 cells and myotubes. The constructed muscular tissue specificity expression system has independent starting activity and muscular tissue specificity and is capable of adjusting and controlling the expression of the target genes under the effects of induction time and induction dosage.
Year: 2014
Country: CN
Doc No: 103923924