23 Jul PICHIA PASTORIS 2-2 YEAST STRAIN AS PRODUCER OF HUMAN PLATELET GROWTH FACTOR (PDGF-BB) AND METHOD FOR PRODUCTION OF HUMAN PLATELET GROWTH FACTOR
Abstract
FIELD: gene engineering, in particular preparation based on PDGF-BB, useful in therapy, veterinary, diagnosis, tissue culturing. ^ SUBSTANCE: invention relates to PDGF-BB production using Pichia pastoris 2-2 strain as producer of human platelet growth factor. Produced two-dimensional PDGF-BB form is characterized with purity of 95 %, specific activity of 3-5 ED50/ng, and expression level in yeast up to 0.1 g/l of culture. ^ EFFECT: two-dimensional PDGF-BB form with high purity, specific activity and expression level in yeast. ^ 5 cl, 11 dwg, 5 ex
Year: 2006
Country: RU
Doc No: 2290434