23 Jul Pearl sacs assistant formation method after nucleus-insertion
Abstract
The present invention discloses a pearl sac assistant forming method after transplanting nuleus, which comprise the following steps: (1) cutting front segment mantle tissue of pearl oyster (2) using protease for enzymolysis of mantle tissue under temperature 20-25 degree, pH value 6.9-8.3, dispersing the tissue and obtaining cell (3) separating the obtained cell in step (2) by high speed centrifugal apparatus, cell fluid is obtained; (4) neutralizing the cell liquor, (5) immersing neutralized cell fluid in protection liquid; (4) taking optimum volume of cell liquor, dropping it at the wound of transplant operation. The cell liquid integrated with mantle and pearl nucleus to form pearl sac. The present invention successfully operate the cell engineering method in pearl cultivation that cell sac forming time is shortened 10 days, pearl quality is obviously promoted as compared to normal pearl breeding method which largely reduce the formation rate of abnormal pearl and nude pearl without pearl layer.
Year: 2007
Country: CN
Doc No: 101036450