23 Jul Peanut diacylglycerol acyltransferase AhDGAT3 promoter as well as preparation method and application thereof
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Abstract
The invention discloses a peanut diacylglycerol acyltransferase AhDGAT3 promoter (P) as well as a preparation method and application thereof. The nucleotide sequence of the promoter is shown in SEQ ID No.1. A (P) sequence is cloned through a genome walking method, genome DNA is extracted through an SDS cracking process, endonuclease is used for enzyme digestion of DNA in different systems, after DNA is connected with a joint fragment, first PCR amplification is carried out by taking GSP1 and AP1 as primers, and secondary PCR amplification is carried out by taking GSP2 and AP2 as primers, so that a DNA sequence containing (P) is obtained. The promoter has the function of driving exogenous gene expression, the expression part of the promoter is in an embryo of a young and tender seed, the promoter is a tissue specific expression promoter, can drive a target gene to express and accumulate in the embryo of the young and tender seed when being applied to transgenic engineering, and avoids unnecessary waste in a plant metabolic process, therefore, the promoter has a good application prospect in the plant transgenic engineering.
Year: 2015
Country: CN
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