23 Jul Peanut AhFAD2-2A gene promoter and application of peanut AhFAD2-2A gene promoter
Abstract
The invention discloses an AhFAD2-2A gene promoter (P< AhFAD2-2A >) and an application of the AhFAD2-2A gene promoter, and belongs to the technical field of biology. A nucleotide sequence of the promoter is shown as SEQ ID NO.1. The AhFAD2-2A gene promoter is cloned from peanuts, and is used for building a recombinant expression vector; then, the recombinant expression vector is transformed into arabidopsis thaliana by an agrobacterium-mediated transformation method; a downstream recombinant gene is started to be expressed in seeds, roots and anthers, and is not expressed in other tissues; and the promoter belongs to a tissue specificity promoter, so that the promoter can be applied to transgenic engineering; a target gene expression product is accumulated in a certain organ or tissue; the expression amount in the tissue is increased; a good effect is achieved; meanwhile, the self energy waste of plants is avoided; and important application values are realized in the genetic engineering breeding and transgenic research process.
Year: 2015
Country: CN
Doc No: 105087587