23 Jul Oligomeric or polymerized methylene protein and its separation and purification process and use
Abstract
The present invention relates to oligomeric or polymerized subunit protein, its separation and purification process capable of maintaining its activity and resulting in high yield and use. the oligomeric or polymerized subunit protein has two or more independent subunits polymerized on the intermolecular force. Through adding protecting agent of 0.5-40 wt% of vol% concentration into cell culture liquid, animal, plant tissue extracting liquid, crushing cell supernatant, crushed cell balancing liquid or crushed cell eluting liquid at normal temperature and convenient cell crushing , membrane separation, adsorption or chromatographic separation to purify, oligomeric or polymerized subunit protein is obtained. The protein is used in the process of preparing agent engineering protein or vaccine.
Year: 2003
Country: CN
Doc No: 1436804