23 Jul Novel porous fibrin scaffold
Abstract
The invention relates to a novel porous fibrin scaffold, belonging to the technical fields of medical science and tissue engineering. Brief description of the drawings figure 1 is a schematic diagram of an acellular and matrix-free porous pulmonary artery fibrin scaffold. Summary of the invention is that the middle layers of a normal pulmonary arterial wall and a valve are composed of cells, matrixes and fibers. The fibers are main factors for maintaining the biomechanical characteristics of the pulmonary arterial wall and the valve, the cells exist in fiber gaps, and the peripheries of the cells are connected with the fibers by means of the matrix ingredients. A thorough study on the organizational structure of a porcine pulmonary artery is carried out, and the result proves that the reasons of generating rejection reaction, calcification, thrombosis formation and unsatisfactory mechanical properties when directly implanting an acellular porcine pulmonary artery in vivo are as follows: 1. only cell ingredients can be removed by the traditional decellularization method, and the residual matrix ingredients still have certain immunogenicity to induce immunologic rejection to further lead to tissue calcification and decline; and 2. Heterogeneous fibers still have immunogenicity and can still induce the immunologic rejection after being implanted in vivo. To effectively solve the above problems, matrix removal treatment is carried out on the basis of the decellularization to form a pure porous pulmonary artery fibrin scaffold.
Year: 2015
Country: CN
Doc No: 104436300