23 Jul NOVEL G PROTEIN-COUPLED RECEPTOR PROTEINS
Abstract
Regarding the field of genetic engineering, novel G protein-coupled receptor family proteins SREB1, SREB2 and SREB3 expressed in the central nervous system; genes encoding these proteins; and a screening method, etc., with the use of these proteins. An example of methods for acquiring such a G protein-coupled receptor protein as described above comprises effecting RT-PCR with the use of mRNA extracted from a human or rat brain tissue or cells originating in the brain as a template by employing two primers between which the whole translational region of the G protein-coupled receptor protein or a part thereof is sandwiched to thereby obtain cDNA of the G protein-coupled receptor protein or a part thereof and then integrating the cDNA into an appropriate vector followed by the expression thereof in host cells.
Year: 1999
Country: WO
Doc No: 9946378