23 Jul NOVEL G PROTEIN-COUPLED RECEPTOR PROTEINS
Abstract
This invention belongs to the genetic engineering field, and provides novel G protein-coupled receptor family proteins SREB1, SREB2 and SREB3 expressed in the central nervous system, genes coding for said proteins, vectors containing said genes, host cells containing said vectors, processes for producing said G protein-coupled receptor proteins, screening methods using said G protein-coupled receptor proteins, antibodies for said G protein-coupled receptor proteins, and screening methods using said antibodies. Representative method for obtaining the G protein-coupled receptor proteins of the present invention: The reverse transcriptase-polymerase chain reaction (to be referred to as RT-PCR hereinafter) is used for obtaining the G protein-coupled receptor proteins of the present invention. mRNA is extracted from human or rat brain tissue or brain-derived cells. Then, using the mRNA as the template and using two primers interposing the entire portion or a part of the G protein-coupled receptor protein translation region, RT-PCR is carried out to obtain cDNA corresponding to the G protein-coupled receptor protein or a part thereof. Then, the resulting cDNA of the novel C protein-coupled receptor protein or a part thereof is ligated into an appropriate expression vector and expressed in a host cell to produce said G protein-coupled receptor protein.
Year: 2001
Country: EP
Doc No: 1067183